A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus In Vitro

African swine fever virus (ASFV) has been identified as the agent of fever, resulting in a mortality rate nearly 100% domestic pigs worldwide. Protein p22 encoded by gene KP177R reported to be localized at inner envelope virus, while function remains unclear. In this study, interacting proteins host were high-throughput method and analyzed Gene ontology terms Kyoto Encyclopedia Genomes (KEGG) pathways; numerous cellular 293-T that interacted with protein identified. These related biological processes binding, cell structure, signal transduction, adhesion, etc. At same time, participated several KEGG pathways like ribosome, spliceosome, The key protein–protein interaction network closely actin filament organization movement, affecting process phagocytosis endocytosis. A large number identified, providing database, which should very useful elucidate near future, laying foundation for elucidating mechanism ASFV.